Buffer Action | CHEMISTRY | JEE | Concept of the Day | SM Sir
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TAE buffer (IX, working dilution) is: 40 mM Tris base, 1.14% acetic acid (v/v), mM EDTA. Calculate how much a) Tris, b) acetic acid, c) EDTA stock solution and d) water you would need to make 1L of 50X TAE; The molecular weight of Tris is 121.14 g/mol, and your stock solution of EDTA is 0.5 M,
TAE buffer (IX, working dilution) is: 40 mM Tris base, 1.14% acetic aci…
Nov 15, 2022
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How to Prepare a Tris Buffer Solution Tris-hydroxymethyl aminomethane (TRIS) is widely used for the preparation of buffers in biochemical research. Shown here is the structure of TRIS in its protonated form: ^ NH3 HOCH2- C-CH2OH | CH2OH Its acid dissociation constant, Kα, is 8.32 ×10^-9 M. You have available at your lab bench a 0.1 M solution of TRIS in its protonated form, 0.1 M solutions of HCl and NaOH, and ample distilled water. Describe the preparation of a 1-L soluti
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