Enzyme purification using two-phase aqueous partitioningLeucine... | Filo

Plasmid Purification: Isolation and Purification of Plasmid DNA

A biochemist discovers and purifies a new enzyme, generating the purification table below. (a) From the information given in the table, calculate the specific activity of the enzyme after each purification procedure. (b) Which of the purification procedures used for this enzyme is most effective (i.e., gives the greatest relative increase in purity)? (c) Which of the purification procedures is least effective? (d) Is there any indication based on the results shown in the table that the enzyme af