Buffer Action | CHEMISTRY | JEE | Concept of the Day | SM Sir

You are preparing a reaction buffer for the alkaline phosphatase assay buffer. Your lab wants to save money and decides to make the assay buffer in-house rather than ordering premade buffer.You find a recipe that reads:Alkaline Phosphatase Assay Buffer50 mL Recipe:1. MgCl2 Final Concentration: 5 mM2. Tween-20 Final Concentration: 0.1%3. NaCl Final Concentration: 100 mM4. Fill with H2O up to 50 mLYou go to your reagent shelf and find the following stock solutions:1. MgCl2 Concentration: 1M

Making buffer solutions: Video, Causes, & Meaning | Osmosis