Basic Molecular Biology: PCR and Real-Time PCR – Principle of PCR

Choose the Right DNA Polymerase for Your PCR

Polymerase Chain Reaction (PCR)

Biology on Instagram: "The transcription and translation of genetic information are the two main steps of gene expression. During transcription, the enzyme RNA polymerase copies a gene’s DNA sequence into messenger RNA (mRNA) by pairing complementary RNA nucleotides with the DNA template strand, replacing thymine (T) with uracil (U). Next, during translation, the mRNA travels to the ribosome, where transfer RNA (tRNA) molecules read the mRNA codons and bring the corresponding amino acids. These

Dr. Ali -PhD || PU 🇮🇳 on Instagram: "DNA replication is a vital process in which DNA polymerase enzymes create an identical copy of the cell’s genetic material before cell division. At the replication fork, the DNA double helix unwinds and each original strand acts as a template. DNA polymerase adds new complementary nucleotides to form the daughter strands continuously on the leading strand and in short segments called Okazaki fragments on the lagging strand. This enzyme also performs proofre

Ashish Singh on Instagram: "During transcription, the process of converting DNA into RNA involves a change in the base pairing rules. DNA and RNA both use four types of nucleotide bases, but RNA differs slightly because it contains uracil (U) instead of thymine (T). When RNA polymerase reads the DNA template strand, it forms a complementary RNA strand based on specific pairing rules: adenine (A) in DNA pairs with uracil (U) in RNA, thymine (T) pairs with adenine (A), cytosine (C) pairs with guan