RNA Extraction Method (Guanidinium Thiocyanate-Phenol Method) The extraction of RNA is an essential and basic step in any molecular biology experiment such as RT-PCR, cDNA library construction, sequencing, gene expression studies, microarray analysis, etc. There are different types of methods available like CsCl density gradient method, silica column based method but the guanidinium thiocyanate or guanidium isothiocyanate-phenol-chloroform method is the most important and is the common one. Now

Researchers believe there are at least two active chemicals in cannabis that have medicinal applications: cannabidiol and tetrahydrocannabinol. Cannabidiol, also known as CBD, impacts the brain without a high, and tetrahydrocannabinol, also known as THC, has pain relieving properties. Tetrahydrocannabinol (THC) is the main psychoactive cannabinoid found in cannabis, and causes the sensation of getting “high” that’s often associated with marijuana. However, unlike THC, CBD is not psychoactive. #P