Fluorophore fundamentals for flow cytometry

SOLVED: The fluorescence quantum yield of a fluorophore is defined as:#photons emitted / #photons absorbedIt is possible to express it in terms of the rates of the radiative (kr) and nonradiative (knr) decay processes taking place within the molecule. The kinetics of electronic excitation and decay can be modeled as:S0 -> S1kr knrS1 -> S0Here, kr is the radiative decay rate, knr is the nonradiative decay rate, and kA is the photon absorption rate. Assuming that each of these processes follows

Expanding ERF (Equivalent Number of Reference Fluorophore) Value Assignments for Quantitative Flow Cytometry- More Colors and Smaller Particles - Scientific Videos | Thermo Fisher Scientific US