Sharah Capinpin on Instagram: "Stop aggressively pipetting your cells into submission 🙅🏻‍♀️ After centrifugation, cells tend to pack TIGHTLY in a pellet = surface area exposed to buffer drops ⬇️. This prevents buffer (ie media, PBS, lysis buffers etc) to evenly come into contact with the cells. This can result in uneven mixing, poor recovery and inconsistent yield for downstream work like RNA or protein extraction. Plus, adding the buffer directly to a packed pellet can cause it to dislodge as