Multiple sequence alignment (MSA) is a cornerstone of computational biology, facilitating the exploration of functional, structural and evolutionary relationships among biological sequences.

The advent of ultra-high-throughput sequencing technology has captured the imagination of the biological sciences, and with good reason. Ten years ago, on 23 November 1999, the publicly funded human ...

A FASTQ file is a text file that stores the sequence data from clusters that pass the flow cell's filter. Demultiplexing is the first phase in creating a FASTQ file if specimens were multiplexed.

The classic methodology of inferring a phylogenetic tree from sequence data is composed of two steps. First, a multiple sequence alignment (MSA) is computed. Then, a tree is reconstructed assuming the ...