The structure of a key enzyme involved in protein production is revealed by cryo-electron microscopy (cryo-EM). Using cryo-EM, researchers at Goethe University (Frankfurt, Germany) shed light on ...

Figure 1: Three-dimensional reconstruction of the 70S E. coli ribosome in complex with aa-tRNA and EF-Tu. Figure 2: Position of the P-site tRNA. Figure 3: Ternary complex in the A site. Density for ...

In cells transfer RNA (tRNA) translates genetic information from the encoding messenger RNA (mRNA) for protein synthesis. New results from ultrafast spectroscopy and in-depth theoretical calculations ...

In translation, aa-tRNA, EF-Tu and GTP form a ternary complex that binds to the ribosome, allowing codon-anticodon pairing in the 30S ribosomal subunit. Cognate codon recognition triggers fast GTP ...

In all living organisms, the biomolecule transfer RNA (tRNA) plays a fundamental role in protein production. tRNAs are generated from precursor molecules in several steps. The enzyme tRNA splicing ...

The ribosome selects a correct transfer RNA (tRNA) for each amino acid added to the polypeptide chain, as directed by messenger RNA. Aminoacyl-tRNA is delivered to the ribosome by elongation factor Tu ...

Archaeosine is synthesized via three steps in tRNA. ArcS, the second-step enzyme, transfers a lysine to preQ 0, which is introduced into tRNA by ArcTGT, the first-step enzyme. The resultant preQ 0-Lys ...

Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 27 (Jul. 3, 2007), pp. 11268-11273 (6 pages) Pyrrolysine (Pyl), the 22nd natural amino acid and ...

Many viruses have genomes made of RNA. In human cells, messenger RNA (mRNA) molecules act as intermediates; cellular machinery copies a DNA sequence into mRNA so that it can then be translated into a ...

Archaeosine is a modified nucleoside found only in the tRNAs from archaea, the so-called third domain of life, and contributes to the maintenance of the L-shaped tRNA three-dimensional structure. The ...