You can subtract multiple cells from one cell in Excel by using Minus sign, SUM function and Paste Special feature. Here is a tutorial on this.

You can subtract in Excel by placing a minus sign in front of any numbers you want to deduct. You can add and subtract a bunch of numbers at once using the equal sign or SUM function.

Subtracting isn’t as straightforward in Excel as it seems, simply because there’s no actual command to subtract numbers. Instead, you have to use a formula with the subtraction operator.