In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...

Total internal reflection microscopy is a light microscopy technique that relies on total internal reflection of illumination light to generate an evanescent wave that extends less than 200 nm from ...

Total internal reflection–fluorescence correlation spectroscopy (TIR-FCS) is an emerging technique that is used to measure events at or near an interface, including local fluorophore concentrations, ...

Science presenter Jon Chase explains total internal reflection. He demonstrates how total internal reflection can make a coin disappear inside a glass of water and bend a laser beam. The second ...

Refraction close refractionProcess by which a wave changes speed and sometimes direction upon entering a denser or less dense medium, eg a light ray changes direction when refracted by a lens. from a ...

When we first saw "interactive touch-screen display" in the tips jar, it made us think of playing MegaTouch 9000 at a seedy bar and we were about to toss it in the ol' recycling bin. Then we decided ...